1. Scientists on campus can to be trained to use the FACSCalibur. Please contact chassel@indiana.edu to schedule training and to discuss your needs. She can help you determine which machine is best to do your analysis on. You may also choose to have the specialist run samples for you, therefore bypassing the need for training.
2. The FACSAria (high speed cell sorter) and COPAS Select (Drosophila embryo sorter) must be run exclusively by our trained specialist. Users will schedule a time for the specialist to run samples. This will be done via email, but available times will be posted on the website.
3. The facility is classified as a BSL2 facility. Therefore, regardless of the type of material you work with, all those using the facility will be required to attend a BSL2 biosafety training provided by the facility. Please contact Christiane Hassel to schedule a training session (these training sessions are free).
4. A fee will be charged for anyone who signs up and fails to notify the center that they no longer intend to use the time slot. A 12 hour minimum notification to cancel your time slot is required. Unusual or extenuating circumstances for last minute notifications will be reviewed on a case by case basis.

While the FCCF has many well established protocols for flow cytometry in place, we understand that members of the IU flow community may be experimenting with methods untried in the facility. New methods, along with new cell types involved in these methods, may necessitate some troubleshooting to establish the best protocol and conditions for your cell type. For those researchers who are using methods new to the facility and for labs new to the university, we offer a discounted “new method” and “new lab” fee for the first 6 hours (or approximately 3 sessions) of training and flow cytometer use. Please see the user fees below for the “new method” and “new lab” rates.

As of July 1, 2008, the following fee schedule has been implemented for the use of all FACS machines:

New Method / New Lab rates as follows:

FACSCalibur
$25 per hour (if user runs it)
$40 per hour (training and tech-assisted runs)

FACSAria
$40 per hour (samples must be run by the specialist)

Regular rates as follows:

FACSCalibur
$50 per hour (if user runs it)
$80 per hour (training and tech-assisted runs)

FACSAria
$80 per hour (samples must be run by the specialist)

COPAS Select
cost TBD