IU seal
Indiana University Bloomington
Flow Cytometry Core Facility
rainbow laser beam
Protocols & Reagents

Protocols

These protocols have been used by researchers at IU Bloomington:

Additional assays and sorting performed by researchers at IU Bloomington:

  • Drosophila embryo sorting
  • Intracellular marker staining
  • Measurement of Green Fluorescent Protein (GFP) expression and sorting
  • Mitochondrial membrane potential
  • Single Cell Sorting
  • Surface marker Staining and sorting
  • Viability assays

Other assays available:

  • Bacterial counting
  • BrdU incorporation
  • Cell proliferation assays
  • Chromosome analysis and sorting
  • Intracellular Cytokine detection
  • Multiparameter Immunofluorescence analysis and cell sorting
  • Signal Transduction
  • and much more!
Other resources for cell preparation and staining for flow cytometry

Reagents

FACS Products Available in the Biology Stockroom

Product

Packaging

Location

Cost

Instrument

 

BD Falcon Tubes w/ cap
12x75mm
Ref# 352054

 

125/package
Sterile

 

Stockroom

 

$13.96 package

 

Aria
Calibur

 

BD Falcon Tubes
12x75mm

 

Individually wrapped
Sterile

 

Stockroom

 

$0.17 each

 

Aria ONLY

 

BD Falcon Tubes w/ cap
17x100mm

 

Individually wrapped
Sterile

 

Stockroom

 

$0.16 each

 

Aria ONLY

 

BD Falcon Cell strainers - 40um
Ref# 352340

 

Individually wrapped

 

Stockroom

 

$1.74 each

 

Aria
Calibur

 

Plastic container – 12qt w/ Lid

 

N/A

 

Stockroom

 

$4.29 each

 

N/A – for carrying samples to FCCF

 

Plastic container – 19qt w/ Lid and snap closures

 

N/A

 

Stockroom

 

$7.49 each

 

N/A – for carrying samples to FCCF

 

Disposable Lab coat (various sizes)

 

 

N/A

 

Stockroom

 

M - $2.58 each
L - $2.75 each
XL - $2.48 each

 

Aria
Calibur

 

Safety Glasses

 

Box

 

Stockroom

 

$3.94 each

 

Aria
Calibur

 

Gloves

 

Box

 

Stockroom

 

Various types, sizes, prices available

 

Aria
Calibur