Flow cytometry sorter with FACSDiva software

The FACSAria is high speed cell sorter that can sort up to 70,000 events per second and can analyze up to 15 parameters.  The IU Bloomington FACSAria is a custom build unit that includes:

• 4 lasers: Blue – 488nm, Red – 633nm, Violet – 407nm, and UV – 355nm. 
• 2 nozzles sizes: 70um and 100um
  
• Aerosol management system
  
• Temperature control system
• 2 and 4 way sorting
  • 2 way sorting into 5mL or 15mL tubes
  • 4 way sorting into 5mL or 1mL tubes

It has an Automated Cell Deposition Unit (ACDU) designed for sorting into multiwell plates or onto microscope slides.

More information can be found at: http://www.bdbiosciences.com/pdfs/brochures/SJ-0003-00.pdf

The FACSAria is run by our dedicated flow cytometry technician, Christiane Hassel.  Users may not run this equipment themselves. 

Cost for usage: $80/hr

Flow cytometry analyzer with CellQuest Pro software

The FACSCalibur is a 2 laser analyzer, capable of multicolor analysis of up to 4 colors.  The FACSCalibur includes the following:

2 lasers: Blue – 488nm and Red – 633nm
Sample injection port (SIP) diameter: 150um
Automated loader function
Sort option (low speed)

More information can be found at: http://www.bdbiosciences.com/pdfs/brochures/23-3090-03.pdf

FACSCalibur training is available and users may self-run this instrument after a training period.  Alternatively, users may have our dedicated technician run samples for them.

Cost:		$50/hr – user run
		80/hr – training, technician run

Flow cytometry large particle sorter

The COPAS Select is a large particle sorter, which in highest sort mode, can sort from 10-15 events per second.  It can sort C. elegans, Drosophila, plant seeds and cells, and beads, and can perform multicolor analysis of up to 3 colors.

The COPAS Select at the IUB FCCF has these specifications:

• 1 laser: Blue laser – tunable to 488 or 514nm excitation, can detect Green, Yellow, and Red emission wavelengths (510, 545, and 580nm)
• Flow cell size:  500µm – can collect events from 20-350µm in diameter
• 1 way sorting: into collection device
• X-Y stage: for sorting into 24 or 96-well plates

More information can be found at: http://unionbio.com/products/select.html

The COPAS Select is run by a dedicated flow cytometry technician – please contact us if you are interested in having samples run.

Cost for usage:  TBD

The Z2 instrument is a cell and particle counter that reports both cell counts and concentration of a cell population, as well as size distribution of that cell population. More information about the Z2 Coulter Counter can be found at: http://www.beckman.com/products/instrument/partChar/pc_z2.asp

Cost: TBD

More information to come soon!

Please contact the facility(email) for more about the Z2 and using this instrument.