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Indiana University Bloomington
Flow Cytometry Core Facility
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Welcome to the IUB Flow Cytometry Core Facility

The IU Bloomington Flow Cytometry Core Facility (FCCF) offers flow cytometry analysis and cell sorting services. It houses the FACS Aria II, a state-of-the-art sorter/analyzer capable of analyzing fourteen colors and seventeen parameters, the COPAS Select, a Drosophila embryo and large particle sorter, the LSRII, a thirteen color, sixteen parameter analyzer with HTS option, the FACSCalibur, a four-color analyzer, as well as Z2 Coulter counter technology used for determining cell concentration and size distribution. The facility also houses the AmpliGrid system (discontinued) for single-cell PCR. Services include technician assisted sorting, as well as training for analyzers, PCR and Coulter instruments. Help with experimental design is also available. The facility maintains a workstation with flow cytometry data analysis software, and also has a copy of this software for use outside of the facility. For more information and for any questions, please contact the facility.


April 9, 2014 IU Bloomington Newsroom article - "IU Bloomington faculty awarded awarded Indiana Clinical and Translational Sciences grants" (click on title to read article)

Fall 2014 - the FCCF will be offering an Introduction to Flow Cytometry Z620 course for the second time (8-week course)

Indiana CTSI funding - Spring 2014 core pilot funding for use of CTSI designated core facility is due Thursday May 1 by 4pm: https://www.indianactsi.org/grantinfo/grant/162

Upcoming seminar November 6, 2013 - Jerry Aultz from EMD Millipore will present on the Amnis FlowSight Imaging Flow Cytometer

Next semester (Spring 2014) - the FCCF will be offering an Introduction to Flow Cytometry Z620 course

August 16, 2013 - The FlowJo traveling dongle was upgraded to support the newest version of FlowJo, vX. The Windows workstation runs v7.6.5. See more information about using FlowJo on the Data Analysis page of the website.

Reminder (April 10, 2013): for those budgeting for grants and other funding, facility rates will increase July 1, 2013 for all instrumentation. Rate information can be found in the following [PDF]




>> The IUB FCCF is a CTSI designated core - this means that researchers may apply for funds that can be used to perform research with facility equipment. More information about funding opportunities can be found at https://www.indianactsi.org/ - go to "Grants and Funding"

>> The FCCF is on Facebook - find and like us at http://www.facebook.com/pages/IUB-FCCF/231178580343589 - you do not need to be a member of Facebook to view this (just click on "close" in the bottom righthand corner of the box that pops up)


Oversight Committee

John Foley, Ph.D. (Med Sci) - Director
Kris Klueg, Ph.D. (CGB) - Associate Director
Christiane Hassel, M.S. (Biology) - Manager
Christina Dann, Ph.D (Chemistry)
Ke Hu, Ph.D. (Biology)
Roger Innes, Ph.D. (Biology)
Thom Kaufman, Ph.D. (Biology)
Melanie Marketon, Ph.D. (Biology)
Ken Nephew, Ph.D (Med Sci)