Welcome to the IUB Flow Cytometry Core Facility
The IU Bloomington Flow Cytometry Core Facility (FCCF) offers flow cytometry analysis and cell sorting services. It houses the FACS Aria II
, a state-of-the-art sorter/analyzer capable of analyzing fourteen colors and seventeen parameters, the COPAS Select
, a Drosophila
embryo and large particle sorter, the LSRII
, a thirteen color, sixteen parameter analyzer with HTS
option, the FACSCalibur
, a four-color analyzer, as well as Z2 Coulter counter
technology used for determining cell concentration and size distribution. The facility also houses the AmpliGrid
) for single-cell PCR. Services include technician assisted sorting, as well as training for analyzers, PCR and Coulter instruments. Help with experimental design is also available. The facility maintains a workstation with flow cytometry data analysis software, and also has a copy of this software for use outside of the facility. For more information and for any questions, please contact the facility
Fall 2014 - the FCCF is offering an Introduction to Flow Cytometry Z620 course
for the second time (8-week course)
Space is limited!
July 2014 - July 7: Foot pedals were successfully installed at the flow facility sink: please use foot pedals for hand washing purposes, July 11: an extra faucet was added to the sink for decontaminated waste disposal; this faucet should NOT be used for hand washing, July 14: the annual IU-B Flow Core User Satisfaction survey will be emailed to the facility listserv (survey deadline is August 15, 11:59pm)
- flow facility survey can be found at: IU-B Flow Core Users Survey 2014
Cyto 2014 - meeting updates: check the Facebook page for information http://www.facebook.com/pages/IUB-FCCF/231178580343589 (no Facebook account needed!)
May 29, 2014 - The Calibur workstation was upgraded from OS9 to OSX. All files/templates still on the OS9 workstation were transferred.
May 2014 - Two FlowJo dongles are now available for check out and are version X. The facility workstation was upgraded to Windows 7 and runs FlowJo vX
April 9, 2014 IU Bloomington Newsroom article - "IU Bloomington faculty awarded awarded Indiana Clinical and Translational Sciences grants"
(click on title to read article)
Indiana CTSI funding - Spring 2014 core pilot funding for use of CTSI designated core facility is due Thursday May 1 by 4pm: https://www.indianactsi.org/grantinfo/grant/162
>> The IUB FCCF is a CTSI designated core - this means that researchers may apply for funds that can be used to perform research with facility equipment. More information about funding opportunities can be found at https://www.indianactsi.org/ - go to "Grants and Funding"
>> The FCCF is on Facebook - find and like us at http://www.facebook.com/pages/IUB-FCCF/231178580343589 - you do not need to be a member of Facebook to view this (just click on "close" in the bottom righthand corner of the box that pops up)
John Foley, Ph.D. (Med Sci) - Director
Kris Klueg, Ph.D. (CGB) - Associate Director
Christiane Hassel, M.S. (Biology) - Manager
Christina Dann, Ph.D (Chemistry)
Ke Hu, Ph.D. (Biology)
Roger Innes, Ph.D. (Biology)
Thom Kaufman, Ph.D. (Biology)
Melanie Marketon, Ph.D. (Biology)
Ken Nephew, Ph.D (Med Sci)