Welcome to the IUB Flow Cytometry Core Facility
The IU Bloomington Flow Cytometry Core Facility (FCCF) offers flow cytometry analysis and cell sorting services. It houses the FACS Aria II, a state-of-the-art sorter/analyzer capable of analyzing twelve colors and fifteen parameters, the COPAS Select, a Drosophila embryo and large particle sorter, the LSRII, an eight-color analyzer, the FACSCalibur, a four-color analyzer, as well as Z2 Coulter counter technology used for determining cell concentration and size distribution. The facility also houses the AmpliGrid system for single-cell PCR. Services include technician assisted sorting, as well as training for analyzers, PCR and Coulter instruments. Help with experimental design is also available. The facility maintains a workstation with flow cytometry data analysis software, and also has a copy of this software for use outside of the facility. For more information and for any questions, please contact the facility.
Update April 2, 2012 - the Flow Facility METACyt proposal for upgrades to the Aria II and LSRII has been funded! Upgrades include yellow-green lasers for the Aria II and LSRII as well as addition of a FSC-PMT and High throughput sampler (HTS) to the LSRII. We anticipate the upgrade to take a couple of months, but will keep everyone updated.
- Starting January 5, 2012, the flow facility will resume normal hours Monday through Friday, 9-5pm.
- There will be times when the facility manager is not available, please see the calendar for these times
Roger Innes, Ph.D. (Biology)
Kris Klueg, Ph.D. (CGB)
Melanie Marketon, Ph.D. (Biology)
Thom Kaufman, Ph.D. (Biology)
Robert "Tank" Eisman, Ph.D (Biology)
Rich Hardy, Ph.D. (Biology)
Ken Nephew, Ph.D (Med Sci)
Curt Balch, Ph.D. (Med Sci)
Christiane Hassel, M.S. (Biology)
Kah Tan-Allen, Ph.D. (Med Sci)
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