Welcome to the IUB Flow Cytometry Core Facility
The IU Bloomington Flow Cytometry Core Facility (FCCF) offers flow cytometry analysis and cell sorting services. It houses the FACS Aria II, a state-of-the-art sorter/analyzer capable of analyzing fourteen colors and seventeen parameters, the COPAS Select, a Drosophila embryo and large particle sorter, the LSRII, a thirteen color, sixteen parameter analyzer with HTS option, the FACSCalibur, a four-color analyzer, as well as Z2 Coulter counter technology used for determining cell concentration and size distribution. The facility also houses the AmpliGrid system ( discontinued) for single-cell PCR. Services include technician assisted sorting, as well as training for analyzers, PCR and Coulter instruments. Help with experimental design is also available. The facility maintains a workstation with flow cytometry data analysis software, and also has a copy of this software for use outside of the facility. For more information and for any questions, please contact the facility.
Reminder (April 10, 2013): for those budgeting for grants and other funding, facility rates will increase July 1, 2013 for all instrumentation. Rate information can be found in the following [PDF]
January 15 - 17, 2013 - COPAS Select upgrade installation. Upgrades include 20mW 488nm laser with new optics, upgraded workstation and COPAS Software, and Advanced Acquisition Package (AAP)
December 5, 2012 (Wednesday) - Rock Pulak from Union Biometrica will give a presentation on the Union Biometrica BioSorter, a large (and small) particle sorter that gently analyzes and sorts cells, organisms, and particles from 1um to 1,500um. Seminar will be held in SI001, 10:30am.
October 2012 - The FCCF is now on Facebook - find and like us at http://www.facebook.com/pages/IUB-FCCF/231178580343589 - you do not need to be a member of Facebook to view this (just click on "close" in the bottom righthand corner of the box that pops up)
July 1, 2012 - New user rates have been implemented - please see the Rates & Policies page for information regarding new rates
June 27, 2012 - Aria II and LSRII upgrades have been completed and instruments have been upgraded as follows:
- Aria II: addition of 150mw 561nm Yellow-Green laser for excitation of a variation of Fruit Proteins and more efficient excitation of PE and PE tandem dyes
- LSRII: addition of a 150mw 561nm Yellow-Green, replacement of 633nm HeNe laser with 150mw 636nm laser, addition of a FSC-PMT for sub-micron particle discrimination and addition of a High-Throughput Sampler (HTS) for sample acquisition and analysis in 96 and 384-well format
Roger Innes, Ph.D. (Biology) - Director
Kris Klueg, Ph.D. (CGB) - Associate Director
Christiane Hassel, M.S. (Biology) - Manager
Curt Balch, Ph.D. (Med Sci)
Robert "Tank" Eisman, Ph.D (Biology)
John Foley, Ph.D. (Med Sci)
Rich Hardy, Ph.D. (Biology)
Thom Kaufman, Ph.D. (Biology)
Melanie Marketon, Ph.D. (Biology)
Ken Nephew, Ph.D (Med Sci)
Kah Tan-Allen, Ph.D. (Med Sci)
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